Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60010-1000.021.JPG-250.jpg)
Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 Liter
TAE 10X Solution is a concentrated Tris-acetate-EDTA buffer used in molecular biology for DNA and RNA electrophoresis. When diluted to 1X, it maintains pH (~8.3) and low ionic strength. It supports clear nucleic acid separation, extended run times, and protects samples from degradation by chelating divalent metal ions like Mg²⁺.
Substance Name: [TRIS Acetate EDTA 10X Solution]
10X solution contains:
TRIS-Base: 0.4 M
Acetic Acid: 0.2 M
EDTA: 10 mM
pH (10X Solution (@25°C): 8.2-8.4
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-1000.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-20 Bis-Tris Preca
mPAGE 4-20% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Research Products International Corp Stacking Buffer 4X Solution, 500 ML
4X concentrated buffer solution for preparation of Laemmli SDS-PAGE resolving gels.
Teknova 10mM Tris, 0.15M Sodium Chloride, 0.05% Tween-20. pH 8.0. 500mL, Sterile.
10mM Tris,0.15M Sodium Chloride,0.05% Tween-20
LAB BIOREAGENTS LowBinding V Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Selleck Chemical LLC P62-mediated mitophagy inducer
P62-mediated mitophagy inducer (PMI) is a P62-mediated activator of mitophagy that functions independently of parkin recruitment or mitochondrial membrane potential ( m) collapse It remains effective even in cells lacking a fully functional PINK1/Parkin pathway PMI stabilizes Nrf2 and enhances p62 expression thereby activating mitophagy
Electron Microscopy Sciences Tris Maleate Buffer 0.2M, pH range 6.4-8.0 500 ML
Please specify pH when ordering.
pH: 6.4-8.0
Teknova 10mM Tris-HCl, pH 8.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
![eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 220598-43-6 | MFCD18073347 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159302.JPG-250.jpg)
eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 220598-43-6 | MFCD18073347 | 1g
Pharmablock | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 1g | 551175136 | PBZ0195 | | 220598-43-6 | MFCD18073347 | 213.277 | C11H19NO3
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