Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC MOPS | 1132-61-2 | 99.9% | 100 G
MOPS is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It also significantly improves the thermal stability of BSA (HY-D0842).
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 250 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0
Sigma Aldrich Fine Chemicals Biosciences BICINE Redi DriTM 99 free 500G
BICINE Redi DriTM 99 free 500G
Medchemexpress LLC HEPES, NaCl, Sucrose, pH 7.2 buffer | 100 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is primarily used to resuspend human erythrocyte ghost preparations.
- Physiological buffer
- Composed of HEPES, NaCl, and Sucrose
- Adjusted to pH 7.2
- Used to resuspend human erythrocyte ghost preparations
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, particularly in the preparation of enzyme buffers.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Especially useful in the preparation of enzyme buffers
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solutin500ML
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Bioworld TTETRIS-TAPS-EDTA) BUFFER
This item has a minimum order quantity of 2 per supplier requirements. TTE (TRIS-TAPS-EDTA) BUFFER POWDER BLEND: - Proteomics Grade - An electrophoresis running buffer for DNA Sequencing that yields longer reads
Biotang Inc Sodium Phosphate Buffer 0.5M, pH7.6, 250 ml
Sodium Phosphate Buffer 0.5M, pH7.6, 250 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml.
Biotang Inc Citrate-buffered Saline or SSC (20X), 1000 ml
Citrate-buffered Saline or SSC (20X), 1000 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 9.0 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 9.0 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.4 (0.2 micron filtered), 500 ml.
eMolecules 502650-56-8 | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g
Pharmablock - Stock | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g | 534153256 | PBJL037 | 502650-56-8 | 172.224 | C9H16O3
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